Bacteriophage libraries containing millions of variants of phage tail fiber motifs on a common structural scaffold give rise to infectious phages with expanded or altered host ranges, which may be useful for
Regions in the T3 tail fiber were targeted for mutagenesis to create highly diverse phage libraries that were screened to identify phages with altered
The correlation of an unstable baseplate and increased ease of contraction with the reduced requirement for tail fiber binding suggests that the binding of the fibers plays an active role in
Tailed phages use a broad range of receptor-binding proteins, such as tail fibres, tail spikes and the central tail spike, to target their cognate bacterial
Together with the nucleic-acid containing capsid, a virus possesses other structures, such as the tails and the fibers, whose physical properties have not been studied yet with single molecule techniques
Organization of the bacteriophage T4 long tail fiber. (A) A structural model of bacteriophage T4 virion showing the head, the tail, and the long tail
Here, we introduce RBPseg, a method that combines monomeric ESMFold predictions with a structural-based domain identification approach, to divide tail fiber sequences into manageable
Here, we introduce RBPseg, a method that combines monomeric ESMFold predictions with a structural- based domain identification approach, to divide tail fiber sequences into
RBPseg enables accurate modeling of tail fiber structure, providing the first comprehensive tail fiber structure atlas.
Recent significant advances at single-molecule and atomic levels have begun to unravel the structural organization of tail fibers and underlying
Advances in synthetic biology may soon allow a bottom-up approach for engineering custom phages or phage tail fibers, but for this to become reality we must first have a better
The system includes both tail fibers and a tail spike. The terminal regions of the tail fiber proteins possess carbohydrate-binding domains and
Because ORF46 and ORF47 are essential for tail formation, as is the TMP (52), we propose that these three proteins form a tail initiator complex constituting the tail fiber and the conical structure, which
Using this approach, we generated complete tail fiber models, validated by single- particle cryo–electron microscopy of five fibers from three phages. A structural classification of 67 fibers
Regions in the T3 tail fiber were targeted for mutagenesis to create highly diverse phage libraries that were screened to identify phages with altered host-ranges. We sought to expand the
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RBPseg workflow in detail, step-by-step demonstrating the 682 architecture of RBPseg using TC14 fiber as example. A FASTA file is input to ESMfold, which 683 generates a monomeric model.
In this case, cell-debris escape presents a paradox: the phage must first bind the surface of a susceptible host cell and then avoid rebinding it during host lysis. The assembly of TFs is
Similar to fiber optic jumpers, tail fibers are classified into single-mode and multimode types, differing in color, wavelength, and transmission distances. Generally, multimode tail fibers are
Author summary Bacteriophage (phage) T4 belongs to myoviridae, a widely distributed family of viruses on Earth. They contain a head (capsid), a
The US Coast Guard said it shows "the aft dome, aft ring, remnants of the hull and carbon fiber debris". It comes shortly after images of the sub''s tail
R pyocins are ɸCTX-like myophage tailocins of Pseudomonas sp. Adsorption of R pyocins to target strains occurs by the interaction of tail fiber proteins with core lipopolysaccharide (LPS).
Our results demonstrated that the tail fibers of P. aeruginosa phages were involved in host recognition, and that they can be replaced with tail fibers
Abstract 1165 Tail fiber chaperone protects bacteriophage P2 from suicidal binding to cell debris during infection John-Mark Miller ∙ Seth Scott ∙ Mark White
In this paper, we introduce RBPseg, a method that combines monomeric ESMfold predictions with a novel sigmoid distance pair (sDp) protein
Viral fibrous protein part of the virion fiber, which is a thin long rod like structure, unique or multiple, appended to the tail of prokaryotic viruses and used for attachment to the host cell. Shorter
Tail fibers are responsible for the specific, albeit reversible primary attachment to host cell.
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